|
KMID : 0613820180280010026
|
|
Journal of Life Science
2018 Volume.28 No. 1 p.26 ~ p.36
|
|
|
Antioxidant and Antidiabetic Activities of Jerusalem Artichoke Composites Containing Gynura procumbens, Momordica charantia, and Curcuma longa via AMPK Activation
|
|
Lee Soo-Jung
Hu Wen-Si
Pyo Jae-Ho
Ryu Ji-Hyeon
Kang Da-Won
Jeong Bo-Young
Sung Nak-Ju
|
|
|
Abstract
|
|
|
|
This study was performed to identify the antioxidant and α-glucosidase inhibitory activities of water and 70% ethanol extracts of the three following herbs: G. procumbens, M. charantia, and C. longa. In addition, the antioxidant and antidiabetic activities of five types of Jerusalem artichoke composites (JA1?5), which were prepared by adding ethanol extracts of several herbs to Jerusalem artichoke concentrate, were studied and compared. The results showed that the total phenol and flavonoid contents of the ethanol extracts were higher than those of the water extracts. The DPPH and ABTS radical scavenging activities and reducing power depended on the total phenol and flavonoid contents. The antioxidant activities of ethanol extracts from G. procumbens and C. longa were comparable. Moreover, the α-glucosidase inhibitory activity of the ethanol extracts (2,000 μg/ml) from each herb was found to be over 50%. In contrast, the five types of JA composites showed higher total phenol and flavonoid contents than those of JA concentrate. In addition, increased antioxidant and α-glucosidase inhibitory activities were observed, with that of JA1 being the highest. However, all concentrations (1~100 μg/ ml) of JA tested did not affect the cell viability of Chang cells. In addition, JA induced the activation of AMP-activated protein kinase (AMPK) in Chang cells and significantly increased the glucose uptake in C2C12 cells. Therefore, it could be concluded that the JA composites (JA1?5) mixed with G. procumbens, M. charantia, and C. longa extracts were effective in increasing the extracts’ antioxidant and antidiabetic activities.
|
|
|
KEYWORD
|
|
|
Antioxidant, antidiabetes, Gynura procumbens, Jerusalem artichoke
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|
|